HSP Beta2 Polyclonal Antibody

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Home > Product > Antibody > HSP Beta2 Polyclonal Antibody

HSP Beta2 Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

HSP beta2 Antibody

Code

BT-AP04245

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human HSPB2. AA range:121-170

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

Heat shock protein beta-2 encoded by HSPB2 belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The protein is expressed preferentially in the heart and skeletal muscle. This protein regulates Myotonic Dystrophy Protein Kinase, which plays an important role in maintenance of muscle structure and function.

Uniprot accession

Human: Q16082; Mouse: Q99PR8; Rat: O35878;

Molecular weight

20233

Gene ID

Human: 3316; Mouse: 69253; Rat: 161476

Synonyms

HSPB2; Heat shock protein beta-2; HspB2; DMPK-binding protein; MKBP

Research area

Signal Transduction

Target protein

HSPB2

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ELISA	1: 40000

Images

Western blot analysis of the lysates from HeLa cells using HSPB2 antibody.

Immunofluorescence analysis of A549 cells, using HSPB2 Antibody. The picture on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using HSP β2 Polyclonal Antibody

Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Western blot analysis of lysates from HepG2 and HeLa cells, using HSPB2 Antibody. The lane on the right is blocked with the synthesized peptide.

Protocols

> Protocol Booklet

References

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

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Lead time: Within one week

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