Id4 Polyclonal Antibody

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Home > Product > Antibody > Id4 Polyclonal Antibody

Id4 Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, ELISA

Product overview

Full product name

Id4 Antibody

Code

BT-AP04331

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human ID4. AA range:1-50

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

ID4 encodes a member of the inhibitor of DNA binding (ID) protein family. These proteins are basic helix-loop-helix transcription factors which can act as tumor suppressors but lack DNA binding activity. Consequently, the activity of the encoded protein depends on the protein binding partner.

Uniprot accession

Human: P47928; Mouse: P41139;

Molecular weight

16622

Gene ID

Human: 3400; Mouse: 15904

Synonyms

ID4; BHLHB27; DNA-binding protein inhibitor ID-4; Class B basic helix-loop-helix protein 27; bHLHb27; Inhibitor of DNA binding 4

Research area

Epigenetics and Nuclear Signaling

Target protein

ID4

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC	1: 100 - 1: 300
ELISA	1: 20000

Images

Western blot analysis of lysates from HepG2 cells, using ID4 Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using Id4 Polyclonal Antibody diluted at 1:500 cells nucleus.

Western blot analysis of the lysates from HeLa cells using ID4 antibody.

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Protocols

> Protocol Booklet

References

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

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Lead time: Within one week

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