mTOR Polyclonal Antibody

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Home > Product > Antibody > mTOR Polyclonal Antibody

mTOR Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

mTOR Antibody

Code

BT-AP05646

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human mTOR. AA range:2415-2464

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

Serine/threonine-protein kinase mTOR encoded by MTOR belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. Serine/threonine-protein kinase mTOR acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene.

Uniprot accession

Human: P42345; Mouse: Q9JLN9; Rat: P42346;

Molecular weight

288892

Gene ID

Human: 2475; Mouse: 56717; Rat: 56718

Synonyms

MTOR; FRAP; FRAP1; FRAP2; RAFT1; RAPT1; Serine/threonine-protein kinase mTOR; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; Mammalian ta

Research area

Cell Biology

Target protein

MTOR

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ELISA	1: 40000

Images

Western Blot analysis of HuvEc cells using mTOR Polyclonal Antibody diluted at 1:2000

Western blot analysis of customer's lysis using mTOR antibody. Antibody was diluted at 1:2000

Immunofluorescence analysis of Hela cell. 1,mTOR Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using mTOR Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from K562 cells, using mTOR Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using mTOR Polyclonal Antibody diluted at 1:2000

Protocols

> Protocol Booklet

References

Customer reviews and Q&As

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

Custom quote | Bulk request

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