N4BP1 Polyclonal Antibody

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Home > Product > Antibody > N4BP1 Polyclonal Antibody

N4BP1 Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human

Applications: WB, IHC-p, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Applications: WB, IHC-p, ELISA

Product overview

Full product name

N4BP1 Antibody

Code

BT-AP05751

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human N4BP1. AA range:383-432

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

N4BP1 is a Protein Coding gene. NEDD4 Binding Protein 1 is a inhibitor of the E3 ubiquitin-protein ligase ITCH. Acts by interacting with the second WW domain of ITCH, leading to compete with ITCHs substrates and impairing ubiquitination of substrates. Gene Ontology (GO) annotations related to this gene include RNA binding. An important paralog of this gene is ZC3H12C.

Uniprot accession

Human: O75113; Mouse: Q6A037;

Molecular weight

100409

Gene ID

Human: 9683

Synonyms

N4BP1; KIAA0615; NEDD4-binding protein 1; N4BP1

Research area

Epigenetics and Nuclear Signaling

Target protein

N4BP1

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC	1: 100 - 1: 300
ELISA	1: 40000

Images

Western blot analysis of lysates from MCF-7 and Jurkat/293 cells, using N4BP1 Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using N4BP1 Polyclonal Antibody cells nucleus.

Western Blot analysis of K562 cells using N4BP1 Polyclonal Antibody cells nucleus.

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Protocols

> Protocol Booklet

References

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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