p47-phox Polyclonal Antibody

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Home > Product > Antibody > p47-phox Polyclonal Antibody

p47-phox Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Monkey

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Monkey

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

p47-phox Antibody

Code

BT-AP06794

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human p47 phox. AA range:281-330

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

The protein encoded by NCF1 (neutrophil cytosolic factor 1) is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in NCF1 have been associated with chronic granulomatous disease.

Uniprot accession

Human: P14598; Mouse: Q09014;

Molecular weight

44683

Gene ID

Human: 653361; Mouse: 17969

Synonyms

NCF1; NOXO2; SH3PXD1A; Neutrophil cytosol factor 1; NCF-1; 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NCF-47K; Neutrophil NADPH oxidase factor 1; Nox org

Research area

Signal Transduction; Metabolism

Target protein

NCF1

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ELISA	1: 20000

Images

Western blot analysis of lysates from COLO205, using p47 phox Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunofluorescence analysis of HeLa cells, using p47 phox Antibody. The picture on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using p47-phox Polyclonal Antibody

Protocols

> Protocol Booklet

References

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

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Lead time: Within one week

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