PPAR-Gamma Polyclonal Antibody

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Home > Product > Antibody > PPAR-Gamma Polyclonal Antibody

PPAR-Gamma Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p

Product overview

Full product name

PPAR-gamma Antibody

Code

BT-AP07357

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

Recombinant Protein of PPAR-γ

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Storage

-20°C for one year

Background

PPARG encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by PPARG is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described.

Uniprot accession

Human: P37231; Mouse: P37238; Rat: O88275;

Molecular weight

57620

Gene ID

Human: 5468; Mouse: 19016; Rat: 25664

Synonyms

PPARG; NR1C3; Peroxisome proliferator-activated receptor gamma; PPAR-gamma; Nuclear receptor subfamily 1 group C member 3

Research area

Epigenetics and Nuclear Signaling

Target protein

PPARG

Recommended dilution

Application	Dilution
WB	1: 500 - 1000
IHC	1: 200 - 500

Images

Immunohistochemical analysis of paraffin-embedded Mouse Heart Tissue using PPAR-γ Polyclonal Antibody.

Western blot analysis of lysates from 1) Hela, 2) 3T3, 3) PC12 cells, (Green) primary antibody was diluted at 1:1000, 4°over night, secondary antibody(cat:RS23920)was diluted at 1:10000, 37° 1hour. (Red) Actin β Monoclonal Antibody(5B7) (cat:YM3028) antibody was diluted at 1:5000 as loading control, 4° over night,secondary antibody(cat:RS23710)was diluted at 1:10000, 37° 1hour.

Western blot analysis of 1) Hela, 2) 3T3, 3) PC12 using PPAR-γ Polyclonal Antibody. Secondary antibody was diluted at 1:20000

Protocols

> Protocol Booklet

References

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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