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Home > Product > Antibody > Ribosomal Protein L27A Polyclonal Antibody

Ribosomal Protein L27A Polyclonal Antibody

Ribosomal Protein L27A Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

Ribosomal Protein L27A Antibody

Code

BT-AP07856

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human RPL27A. AA range:99-148

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL27A encodes a ribosomal protein that is a component of the 60S subunit. 60S ribosomal protein L27a belongs to the L15P family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, multiple processed pseudogenes derived from this gene are dispersed through the genome.

Uniprot accession

Molecular weight

16561

Gene ID

Human: 6157; Mouse: 26451; Rat: 293418

Synonyms

RPL27A; 60S ribosomal protein L27a

Research area

Epigenetics and Nuclear Signaling

Target protein

RPL27A

Recommended dilution

Application Dilution
WB 1: 500 - 1: 2000
IHC 1: 100 - 1: 300
IF 1: 200 - 1: 1000
ELISA 1: 40000

Images

Western blot analysis of lysates from COLO cells, using RPL27A Antibody. The lane on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of A549 cells, using RPL27A Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Western Blot analysis of various cells using Ribosomal Protein L27A Polyclonal Antibody diluted at 1:2000

Protocols

References

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Product size

Price

  • 20ul

    $108.00

  • 50ul

    $180.00

  • 100ul

    $275.00

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Lead time: Within one week

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