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Home > Product > Antibody > Vimentin Polyclonal Antibody

Vimentin Polyclonal Antibody

Vimentin Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

Vimentin Antibody

Code

BT-AP09529

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human Vimentin. AA range:31-80

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

VIM encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. Vimentin encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.

Uniprot accession

Molecular weight

53652

Gene ID

Human: 7431

Synonyms

VIM; Vimentin

Research area

Neuroscience

Target protein

VIM

Recommended dilution

Application Dilution
WB 1: 500 - 1: 2000
IHC 1: 100 - 1: 300
IF 1: 200 - 1: 1000
ELISA 1: 20000

Images

Western Blot analysis of A549 cells using Vimentin Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000
Western Blot analysis of various cells using Vimentin Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000
Immunofluorescence analysis of Hela cell. 1,Vimentin Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Vimentin Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from A549 cells, treated with Nocodazole 1ug/ml 16h, using Vimentin Antibody. The lane on the right is blocked with the synthesized peptide.
Western Blot analysis of various cells using Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000

Protocols

References

Customer reviews and Q&As

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Product size

Price

  • 20ul

    $108.00

  • 50ul

    $180.00

  • 100ul

    $275.00

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Lead time: Within one week

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