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Home > Product > ELISA Primacu > Human Slam Family Member 5, CD84 Primacu™ ELISA Kit

Human Slam Family Member 5, CD84 Primacu™ ELISA Kit

Human Slam Family Member 5, CD84 Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 41.67 pg/mL

Detection range: 156.25-10000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 41.67 pg/mL

Detection range: 156.25-10000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

SLAM family member 5

Code

BPE018

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

41.67 pg/mL

Detection range

156.25-10000 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

The CD2 family receptors are type I transmembrane glycoproteins belonging to immunoglobulin (Ig) superfamily characterized by a membrane-proximal Ig constant 2 (C2) domain and a membrane-distal variable (V) domain that is responsible for ligand recognition. CD84, also known as LY9B and SLAMF5, is a homophilic member of the SLAM (signaling lymphocyte activation molecule) subfamily of the CD2 family. The SLAM family receptorsmediate signal transduction through the interaction of its ITSM (immunoreceptor tyrosine-based switch motifs) in the intracellular region and the SH2 domain of adaptor molecules SAP (SLAM-associated protein) and EAT-2 (EWS-activated transcript 2), and accordingly modulate both adaptive and innate immune responses. The CD84-CD84 interaction was independent of its cytoplasmic tail. Thus, CD84 is its own ligand and acts as a costimulatory molecule. CD84 is expressed on cells from almost all hematopoietic lineages and on CD34+ hematopoietic progenitor cells, suggesting that CD84 serves as a marker for committed hematopoietic progenitor cells.

UniProt accession

MASS(Da)

38782

GeneID

8832

Synonyms

SLAMF5

Gene names

CD84

Research area

Immunology,Stem cells

Target protein

CD84

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE018 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
10000 pg/ml 1.673 1.659 1.551
1.646
5000 pg/ml 1.024 1.012 0.904
1
2500 pg/ml 0.648 0.617 0.509
0.587
1250 pg/ml 0.365 0.368 0.26
0.372
625 pg/ml 0.234 0.238 0.13
0.243
312.5 pg/ml 0.167 0.171 0.063
0.176
156.25 pg/ml 0.14 0.142 0.034
0.144
0 pg/ml 0.103 0.108 0
0.114

Precision

We measured random samples of BPE018 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 632 38 6
Intra-assay 2 20 1620 57.7 3.6
Intra-assay 3 20 5787 253 4.4
Inter-assay 1 20 629 59.5 9.5
Inter-assay 2 20 1625 79.3 4.9
Inter-assay 3 20 5772 265 4.6

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 5*96T

    BPE018

    $2320.00

  • 96T

    BPE018

    $580.00

  • 15*96T

    BPE018

    $6525.00

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Lead time: Within two weeks

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