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Home > Product > ELISA Primacu > Human Plexin Domain-Containing Protein 1, PLXDC1 Primacu™ ELISA Kit

Human Plexin Domain-Containing Protein 1, PLXDC1 Primacu™ ELISA Kit

Human Plexin Domain-Containing Protein 1, PLXDC1 Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 47.93 pg/mL

Detection range: 156.25-10000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 47.93 pg/mL

Detection range: 156.25-10000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Plexin domain-containing protein 1

Code

BPE221

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

47.93 pg/mL

Detection range

156.25-10000 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

Plexin domain-containing protein 1, also known as tumor endothelial marker 3, tumor endothelial marker 7 and PLXDC1 and TEM3, is a secreted, cytoplasm and single-pass type I membrane protein that belongs to the plexin family. PLXDC1 / TEM3 is detected in endothelial cells from colorectal cancer, and in endothelial cells from primary cancers of the lung, liver, pancreas, breast and brain. It is expressed in fibrovascular membrane with increased expression in individuals with proliferative diabetic retinopathy. PLXDC1 / TEM3 is not detectable in endothelial cells from normal tissue. PLXDC1 / TEM3 plays a critical role in endothelial cell capillary morphogenesis. PLXDC1 / TEM3 may play a significant role in the proliferation and maintenance of neovascular endothelial cells in the formation of fibrovascular membranes (FVMs). PLXDC1 / TEM3 may be a molecular target for new diagnostic and therapeutic strategies for proliferative diabetic retinopathy (PDR). PLXDC1 / TEM3 interacts with NID1. It may also interact with CTTN.

UniProt accession

MASS(Da)

55760

GeneID

57125

Synonyms

TEM3;TEM7

Gene names

PLXDC1

Research area

Cardiovascular,Tags & cell markers

Target protein

TEM7

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE221 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
10000 pg/ml 2.946 3.059 2.928
3.172
5000 pg/ml 1.497 1.534 1.403
1.571
2500 pg/ml 0.777 0.765 0.634
0.753
1250 pg/ml 0.44 0.422 0.291
0.404
625 pg/ml 0.277 0.27 0.139
0.263
312.5 pg/ml 0.184 0.196 0.065
0.208
156.25 pg/ml 0.172 0.162 0.031
0.152
0 pg/ml 0.127 0.131 0
0.135

Precision

We measured random samples of BPE221 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 648 33 5.1
Intra-assay 2 20 2700 92.2 3.4
Intra-assay 3 20 6000 224 3.7
Inter-assay 1 20 655 59.6 9.1
Inter-assay 2 20 2703 174 6.5
Inter-assay 3 20 6071 343 5.6

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 5*96T

    BPE221

    $2320.00

  • 96T

    BPE221

    $580.00

  • 15*96T

    BPE221

    $6525.00

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Lead time: Within two weeks

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