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Home > Product > ELISA Primacu > Human Angiopoietin-1 Receptor, T Primacu™ ELISA Kit

Human Angiopoietin-1 Receptor, T Primacu™ ELISA Kit

Human Angiopoietin-1 Receptor, T Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 21.95 pg/mL

Detection range: 78.125-5000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 21.95 pg/mL

Detection range: 78.125-5000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Angiopoietin-1 receptor

Code

BPE123

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

21.95 pg/mL

Detection range

78.125-5000 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

TEK, or TIE-2, is an endothelial cell-specific receptor tyrosine kinase (RTK) that is known as a functioning molecule of vascular endothelial cells. TEK comprises a subfamily of RTK with TIE, and these two receptors play critical roles in vascular maturation, maintenance of integrity and remodeling. Targeted mutagenesis of both Tek and its agonistic ligand, Angiopoietin-1, result in embryonic lethality, demonstrating that the signal transduction pathways mediated by this receptor are crucial for normal embryonic development. TEK signaling is indispensable for the development of the embryonic vasculature and suggests that TEK signaling may also be required for the development of the tumor vasculature.

UniProt accession

MASS(Da)

125830

GeneID

7010

Synonyms

TIE2;VMCM;VMCM1

Gene names

TEK

Research area

Neuroscience,Cardiovascular,Developmental biology,Signal transduction,Stem cells,Cancer

Target protein

TIE2

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE123 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
5000 pg/ml 2.68 2.599 2.508
2.518
2500 pg/ml 1.525 1.5 1.409
1.475
1250 pg/ml 0.83 0.832 0.741
0.834
625 pg/ml 0.432 0.447 0.356
0.461
312.5 pg/ml 0.28 0.267 0.176
0.254
156.25 pg/ml 0.181 0.177 0.086
0.173
78.125 pg/ml 0.143 0.134 0.043
0.125
0 pg/ml 0.094 0.091 0
0.088

Precision

We measured random samples of BPE123 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 266 14.5 5.5
Intra-assay 2 20 1024 47.8 4.7
Intra-assay 3 20 2925 63.4 2.2
Inter-assay 1 20 265 20.5 7.7
Inter-assay 2 20 1015 79 7.8
Inter-assay 3 20 2941 188 6.4

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 5*96T

    BPE123

    $2040.00

  • 96T

    BPE123

    $480.00

  • 15*96T

    BPE123

    $5400.00

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Lead time: Within two weeks

We are continuously updating our ELISA Primacu catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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