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Home > Product > ELISA Primacu > Human Beta-Galactoside Alpha-2,6-Sialyltransferase 1, ST6GAL1 Primacu™ ELISA Kit

Human Beta-Galactoside Alpha-2,6-Sialyltransferase 1, ST6GAL1 Primacu™ ELISA Kit

Human Beta-Galactoside Alpha-2,6-Sialyltransferase 1, ST6GAL1 Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 17.64 pg/mL

Detection range: 78.13-5000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 17.64 pg/mL

Detection range: 78.13-5000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Beta-galactoside alpha-2,6-sialyltransferase 1

Code

BPE207

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

17.64 pg/mL

Detection range

78.13-5000 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

Beta-galactoside alpha-2,6-sialyltransferase 1, also known as B-cell antigen CD75, Sialyltransferase 1, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1, ST6GAL1 and SIAT1, is a single-pass type II membrane protein that belongs to the glycosyltransferase 29 family. Sialyltransferases are key enzymes in the biosynthesis of sialoglycoconjugates that catalyze the transfer of sialic residue from its activated form to an oligosaccharidic acceptor. ST6GAL1 / SIAT1 is normally found in the Golgi but can be proteolytically processed to a soluble form. It is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CDw75, and CD76. β-Galactoside α2,6-sialyltransferases ST6GAL1 and ST6GAL2 are the two unique members of the ST6GAL family described in higher vertebrates. ST6GAL1 / SIAT1 transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates.

UniProt accession

MASS(Da)

46605

GeneID

6480

Synonyms

SIAT1

Gene names

ST6GAL1

Research area

Immunology,Signal transduction

Target protein

ST6GAL1

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE207 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
5000 pg/ml 2.193 2.269 2.134
2.344
2500 pg/ml 1.347 1.278 1.143
1.208
1250 pg/ml 0.741 0.722 0.587
0.702
625 pg/ml 0.434 0.421 0.286
0.408
312.5 pg/ml 0.266 0.263 0.128
0.26
156.25 pg/ml 0.197 0.191 0.056
0.185
78.125 pg/ml 0.161 0.166 0.031
0.171
0 pg/ml 0.126 0.135 0
0.144

Precision

We measured random samples of BPE207 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 180 6.3 3.5
Intra-assay 2 20 562 16.9 3
Intra-assay 3 20 2677 118 4.4
Inter-assay 1 20 180 24.5 13.6
Inter-assay 2 20 558 66.3 11.9
Inter-assay 3 20 2661 283 10.6

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 5*96T

    BPE207

    $2320.00

  • 96T

    BPE207

    $580.00

  • 15*96T

    BPE207

    $6525.00

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Lead time: Within two weeks

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