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Home > Product > ELISA Primacu > Human Dipeptidyl Peptidase 2, DPP7 Primacu™ ELISA Kit

Human Dipeptidyl Peptidase 2, DPP7 Primacu™ ELISA Kit

Human Dipeptidyl Peptidase 2, DPP7 Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 19.9 pg/mL

Detection range: 62.5-4000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 19.9 pg/mL

Detection range: 62.5-4000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Dipeptidyl peptidase 2

Code

BPE128

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

19.9 pg/mL

Detection range

62.5-4000 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

DPP7 (dipeptidylpeptidase 7), also known as DPPII and DPP2, is a post-proline cleaving aminopeptidase expressed in quiescent lymphocytes. Dipeptidyl peptidases (DPPs) have post-proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro dipeptides from the N-termini of proteins. DPPs mediate regulatory activity of their substrates and have been linked to a variety of diseases including type 2 diabetes, obesity and cancer. DPPs can bind specific voltage-gated potassium channels and alter their expression and biophysical properties and may also influence T cells. DPP proteins include DPRP1, DPRP2, DPP3, DPP7, DPP10, DPPX and CD26. It localizes to lysosomes. DPP7 localizes to lysosomes and exists as a homodimer via its leucine zipper motif and is involved in the degradation of oligopeptides. In response to calcium release, it can be secreted in its active form. It is essential for lymphocyte survival, as the inhibition of DPP7 results in quiescent cell apoptosis.

UniProt accession

MASS(Da)

54341

GeneID

29952

Synonyms

DPP2;QPP

Gene names

DPP7

Research area

Signal transduction,Cell biology

Target protein

DPP2

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE128 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
4000 pg/ml 1.56 1.587 1.507
1.614
2000 pg/ml 0.932 0.934 0.854
0.936
1000 pg/ml 0.54 0.529 0.449
0.518
500 pg/ml 0.291 0.308 0.228
0.325
250 pg/ml 0.18 0.184 0.104
0.188
125 pg/ml 0.128 0.132 0.052
0.136
62.5 pg/ml 0.099 0.102 0.022
0.105
0 pg/ml 0.076 0.08 0
0.084

Precision

We measured random samples of BPE128 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 339 24 7.1
Intra-assay 2 20 942 41.4 4.4
Intra-assay 3 20 2244 104 4.6
Inter-assay 1 20 341 26.3 7.7
Inter-assay 2 20 949 60.5 6.4
Inter-assay 3 20 2227 149 6.7

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 5*96T

    BPE128

    $2320.00

  • 96T

    BPE128

    $580.00

  • 15*96T

    BPE128

    $6525.00

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Lead time: Within two weeks

We are continuously updating our ELISA Primacu catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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