Full product name

Code

Assay type

Size

Sensitivity

Detection range

Sample type

Species

Storage

Assay time

UniProt accession

MASS（Da）

GeneID

Synonyms

Gene names

Research area

Target protein

Components	Quantity
Pre-coated ELISA Plate	12 × 8 well strips × 1
Standard Solution	0.2mL × 1 vail
Dilution Buffer	20mL × 1 vail
Stop Solution	6mL × 1 vail
Substrate Solution A	12mL × 1 vail
Substrate Solution B	12mL × 1 vail
Wash Buffer Concentrate (25x)	20mL × 1 vail
Detection Antibody Concentrate	0.6mL × 1 vail

These standard curves of BPE305 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
4000 pg/ml	3.796	3.633	3.522
	3.471
2000 pg/ml	1.978	1.937	1.826
	1.896
1000 pg/ml	0.926	0.962	0.851
	0.998
500 pg/ml	0.524	0.514	0.403
	0.505
250 pg/ml	0.313	0.31	0.199
	0.308
125 pg/ml	0.193	0.205	0.094
	0.218
62.5 pg/ml	0.166	0.16	0.049
	0.155
0 pg/ml	0.114	0.111	0
	0.108

We measured random samples of BPE305 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	20	290	12.4	4.3
Intra-assay	2	20	830	31	3.7
Intra-assay	3	20	2514	95.6	3.8
Inter-assay	1	20	291	38.2	13.1
Inter-assay	2	20	824	80.8	9.8
Inter-assay	3	20	2544	215	8.5

> Protocol Booklet