Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Primacu > Mouse Thrombospondin Type-1 Domain-Containing Protein 1, Thsd1 Primacu™ ELISA Kit

Mouse Thrombospondin Type-1 Domain-Containing Protein 1, Thsd1 Primacu™ ELISA Kit

Mouse Thrombospondin Type-1 Domain-Containing Protein 1, Thsd1 Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 16.94 pg/mL

Detection range: 62.5-4000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 16.94 pg/mL

Detection range: 62.5-4000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Thrombospondin type-1 domain-containing protein 1

Code

BPE315

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

16.94 pg/mL

Detection range

62.5-4000 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8°C

Assay time

3.5h

Background

Thrombospondin type-1 domain-containing protein 1, also known as transmembrane molecule with thrombospondin module, THSD1 and TMTSP, is a single-pass type I membrane protein that contains one TSP type-1 domain. THSD1 is a multi-domain, multi-functional glycoprotein synthesized by many cells. Matricellular THSD1 modulates cell adhesion and proliferation. It is involved in angiogenesis, inflammation, wound healing and cancer. In vitro, nanomolar concentrations of Thrombospondin-1 are required to alter endothelial and vascular smooth muscle cell adhesion, proliferation, motility, and survival. As a major platelet protein, for a long time it was postulated to control hemostasis via platelet aggregate stabilization. THSD1 is a potent angiogenesis inhibitor, and down-regulation of THSD1 has been suggested to alter tumor growth by modulating angiogenesis in a variety of tumor types.

UniProt accession

MASS(Da)

94696

GeneID

56229

Synonyms

Tmtsp

Gene names

Thsd1

Research area

Cardiovascular,Cell biology,Tags & cell markers

Target protein

THSD1

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE315 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
4000 pg/ml 2.417 2.302 2.215
2.188
2000 pg/ml 1.407 1.363 1.276
1.32
1000 pg/ml 0.733 0.772 0.685
0.811
500 pg/ml 0.403 0.421 0.334
0.44
250 pg/ml 0.245 0.252 0.165
0.259
125 pg/ml 0.174 0.165 0.078
0.157
62.5 pg/ml 0.123 0.12 0.033
0.118
0 pg/ml 0.091 0.087 0
0.083

Precision

We measured random samples of BPE315 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 174 11.9 6.8
Intra-assay 2 20 623 28.6 4.6
Intra-assay 3 20 2708 105 3.9
Inter-assay 1 20 173 15.5 8.9
Inter-assay 2 20 616 33.2 5.4
Inter-assay 3 20 2739 191 7

Protocols

References

Customer reviews and Q&As

No reviews
No reviews

Associated products

  • Size

    Code

    Price

  • 5*96T

    BPE315

    $2320.00

  • 96T

    BPE315

    $580.00

  • 15*96T

    BPE315

    $6525.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Primacu catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up