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Home > Product > ELISA Primacu > Human Epidermal Growth Factor Receptor, EGFR Primacu™ ELISA Kit

Human Epidermal Growth Factor Receptor, EGFR Primacu™ ELISA Kit

Human Epidermal Growth Factor Receptor, EGFR Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 8.95 pg/mL

Detection range: 46.88-3000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 8.95 pg/mL

Detection range: 46.88-3000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Epidermal growth factor receptor

Code

BPE004

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

8.95 pg/mL

Detection range

46.88-3000 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently. Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy

UniProt accession

MASS(Da)

134277

GeneID

1956

Synonyms

ERBB;ERBB1;HER1

Gene names

EGFR

Research area

Cancer,Cell biology,Microbiology,Neuroscience,Signal transduction

Target protein

EGFR

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE004 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
3000 pg/ml 2.608 2.683 2.593
2.757
1500 pg/ml 1.529 1.48 1.39
1.43
750 pg/ml 0.78 0.772 0.682
0.763
375 pg/ml 0.414 0.438 0.348
0.461
187.5 pg/ml 0.264 0.261 0.171
0.257
93.75 pg/ml 0.181 0.171 0.081
0.16
46.875 pg/ml 0.135 0.13 0.04
0.124
0 pg/ml 0.091 0.09 0
0.088

Precision

We measured random samples of BPE004 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 138 12.4 9
Intra-assay 2 20 574 21.8 3.8
Intra-assay 3 20 1793 83.5 4.7
Inter-assay 1 20 139 4.3 3.1
Inter-assay 2 20 572 50.9 8.9
Inter-assay 3 20 1776 82.6 4.6

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 5*96T

    BPE004

    $2040.00

  • 96T

    BPE004

    $480.00

  • 15*96T

    BPE004

    $5400.00

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Lead time: Within two weeks

We are continuously updating our ELISA Primacu catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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