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Home > Product > ELISA Primacu > Human Sulfotransferase 1E1, SULT1E1 Primacu™ ELISA Kit

Human Sulfotransferase 1E1, SULT1E1 Primacu™ ELISA Kit

Human Sulfotransferase 1E1, SULT1E1 Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 5.73 pg/mL

Detection range: 31.25-2000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 5.73 pg/mL

Detection range: 31.25-2000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Sulfotransferase 1E1

Code

BPE194

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

5.73 pg/mL

Detection range

31.25-2000 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

Estrogen sulfotransferase, also known as Sulfotransferase, estrogen-preferring, Sulfotransferase 1E1, SULT1E1 and ST1E1, is a cytoplasm enzyme that belongs to the sulfotransferase 1 family. Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. SULT1E1 may control the level of the estrogen receptor by sulfurylating free estradiol. SULT1E1 maximally sulfates beta-estradiol and estrone at concentrations of 2 nM. SULT1E1 also sulfates dehydroepiandrosterone, pregnenolone, ethinylestradiol, equalenin, diethylstilbesterol and 1-naphthol, at significantly higher concentrations; however, cortisol, testosterone and dopamine are not sulfated. SULT1E1 is a key enzyme in estrogen homeostasis. It plays a central role in the prevention and development of human disease.

UniProt accession

MASS(Da)

35126

GeneID

6783

Synonyms

STE

Gene names

SULT1E1

Research area

Metabolism,Signal transduction

Target protein

SULT1E1

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE194 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2000 pg/ml 1.832 1.852 1.732
1.872
1000 pg/ml 1.01 1.054 0.934
1.098
500 pg/ml 0.615 0.589 0.469
0.564
250 pg/ml 0.368 0.37 0.25
0.372
125 pg/ml 0.231 0.242 0.122
0.253
62.5 pg/ml 0.185 0.175 0.055
0.165
31.25 pg/ml 0.159 0.155 0.035
0.151
0 pg/ml 0.129 0.12 0
0.112

Precision

We measured random samples of BPE194 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 69.6 1.9 2.7
Intra-assay 2 20 331 11.8 3.6
Intra-assay 3 20 1004 26.2 2.6
Inter-assay 1 20 69.2 2.7 3.9
Inter-assay 2 20 330 24.7 7.5
Inter-assay 3 20 1012 37.6 3.7

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 5*96T

    BPE194

    $2320.00

  • 96T

    BPE194

    $580.00

  • 15*96T

    BPE194

    $6525.00

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Lead time: Within two weeks

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