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Home > Product > ELISA Primacu > Mouse Carbonic Anhydrase-Related Protein, Ca8 Primacu™ ELISA Kit

Mouse Carbonic Anhydrase-Related Protein, Ca8 Primacu™ ELISA Kit

Mouse Carbonic Anhydrase-Related Protein, Ca8 Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 6.03 pg/mL

Detection range: 31.25-2000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 6.03 pg/mL

Detection range: 31.25-2000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Carbonic anhydrase-related protein

Code

BPE333

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

6.03 pg/mL

Detection range

31.25-2000 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8°C

Assay time

3.5h

Background

The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase protein (CA) VIII, which is a member of the CA gene family, has been shown to have no catalytic CA activity and its biological function is still unknown. Increased expression of CA-RP VIII was observed in 78% of colorectal carcinomas. It suggested that CA-RP VIII plays a role in the process of invasion in colorectal cancer.

UniProt accession

MASS(Da)

33081

GeneID

12319

Synonyms

Cals;Cals1;Car8;Carp

Gene names

Ca8

Research area

Cancer

Target protein

Carbonic Anhydrase 8

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE333 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2000 pg/ml 2.584 2.709 2.575
2.833
1000 pg/ml 1.37 1.43 1.296
1.489
500 pg/ml 0.825 0.772 0.638
0.719
250 pg/ml 0.428 0.447 0.313
0.466
125 pg/ml 0.293 0.291 0.157
0.289
62.5 pg/ml 0.214 0.21 0.076
0.206
31.25 pg/ml 0.168 0.169 0.035
0.17
0 pg/ml 0.133 0.134 0
0.135

Precision

We measured random samples of BPE333 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 197 9.4 4.7
Intra-assay 2 20 506 25.2 5
Intra-assay 3 20 1033 46.2 4.5
Inter-assay 1 20 196 16.3 8.3
Inter-assay 2 20 508 44.1 8.7
Inter-assay 3 20 1036 77.5 7.5

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 5*96T

    BPE333

    $2320.00

  • 96T

    BPE333

    $580.00

  • 15*96T

    BPE333

    $6525.00

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Lead time: Within two weeks

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