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Home > Product > ELISA Primacu > Human Cobalamin Binding Intrinsic Factor, CBLIF Primacu™ ELISA Kit

Human Cobalamin Binding Intrinsic Factor, CBLIF Primacu™ ELISA Kit

Human Cobalamin Binding Intrinsic Factor, CBLIF Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 6.86 pg/mL

Detection range: 15.63-1000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 6.86 pg/mL

Detection range: 15.63-1000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Cobalamin binding intrinsic factor

Code

BPE272

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

6.86 pg/mL

Detection range

15.63-1000 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

Gastric intrinsic factor, also known as GIF, belongs to the of the cobalamin transport protein family. It is a glycoprotein produced by the parietal cells of the stomach. Gastric intrinsic factor plays a key role in the absorption of vitamin B12 on in the small intestine. Vitamin B12 bounds to haptocorrin after entry into the stomach. The resulting complex enters the duodenum, where pancreatic enzymes digest haptocorrin. In the less acidic environment of the small intestine, B12 can then bind to gastric intrinsic factor. This new complex travels to the ileum, where special epithelial cells endocytose them. Inside the cell, B12 dissociates once again and binds to another protein, transcobalamin II. The new complex can exit the epithelial cells to enter the liver.

UniProt accession

MASS(Da)

45416

GeneID

2694

Synonyms

GIF;IFMH

Gene names

CBLIF

Research area

Metabolism,Signal transduction

Target protein

Intrinsic Factor

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE272 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1000 pg/ml 2.774 2.726 2.603
2.678
500 pg/ml 1.497 1.553 1.43
1.609
250 pg/ml 0.866 0.85 0.727
0.833
125 pg/ml 0.503 0.481 0.358
0.458
62.5 pg/ml 0.3 0.3 0.177
0.299
31.25 pg/ml 0.213 0.215 0.092
0.217
15.625 pg/ml 0.169 0.169 0.046
0.168
0 pg/ml 0.117 0.123 0
0.129

Precision

We measured random samples of BPE272 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 60.8 1.5 2.4
Intra-assay 2 20 164 3.5 2.2
Intra-assay 3 20 548 17.7 3.2
Inter-assay 1 20 60.6 4.9 8.2
Inter-assay 2 20 162 13.4 8.3
Inter-assay 3 20 549 39.7 7.2

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 5*96T

    BPE272

    $2320.00

  • 96T

    BPE272

    $580.00

  • 15*96T

    BPE272

    $6525.00

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Lead time: Within two weeks

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