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Home > Product > ELISA Primacu > Human Granulocyte-Macrophage Colony-Stimulating Factor, Csf2 Primacu™ ELISA Kit

Human Granulocyte-Macrophage Colony-Stimulating Factor, Csf2 Primacu™ ELISA Kit

Human Granulocyte-Macrophage Colony-Stimulating Factor, Csf2 Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 4.47 pg/mL

Detection range: 9.38-600 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 4.47 pg/mL

Detection range: 9.38-600 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Granulocyte-macrophage colony-stimulating factor

Code

BPE009

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

4.47 pg/mL

Detection range

9.38-600 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is one of an array of cytokines with pivotal roles in embryo implantation and subsequent development. Several cell lineages in the reproductive tract and gestational tissues synthesise GM-CSF under direction by ovarian steroid hormones and signalling agents originating in male seminal fluid and the conceptus. The pre-implantation embryo, invading placental trophoblast cells and the abundant populations of leukocytes controlling maternal immune tolerance are all subject to GM-CSF regulation. GM-CSF stimulates the differentiation of hematopoietic progenitors to monocytes and neutrophils, and reduces the risk for febrile neutropenia in cancer patients. GM-CSF also has been shown to induce the differentiation of myeloid dendritic cells (DCs) that promote the development of T-helper type 1 (cellular) immune responses in cognate T cells. The active form of the protein is found extracellularly as a homodimer, and the encoding gene is localized to a related gene cluster at chromosome region 5q31 which is known to be associated with 5q-syndrome and acute myelogenous leukemia. As a part of the immune/inflammatory cascade, GM-CSF promotes Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity, and thus worthy of consideration for therapeutic target. GM-CSF has been utilized in the clinical management of multiple disease processes. Most recently, GM-CSF has been incorporated into the treatment of malignancies as a sole therapy, as well as a vaccine adjuvant. While the benefits of GM-CSF in this arena have been promising, recent reports have suggested the potential for GM-CSF to induce immune suppression and, thus, negatively impact outcomes in the management of cancer patients. GM-CSF deficiency in pregnancy adversely impacts fetal and placental development, as well as progeny viability and growth after birth, highlighting this cytokine as a central maternal determinant of pregnancy outcome with clinical relevance in human fertility. Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy

UniProt accession

MASS(Da)

16091

GeneID

12981

Synonyms

Csfgm

Gene names

Csf2

Research area

Immunology,Neuroscience

Target protein

GM-CSF

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE009 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
600 pg/ml 2.338 2.376 2.275
2.415
300 pg/ml 1.36 1.308 1.207
1.256
150 pg/ml 0.678 0.702 0.601
0.727
75 pg/ml 0.402 0.4 0.299
0.399
37.5 pg/ml 0.246 0.235 0.134
0.225
18.75 pg/ml 0.16 0.168 0.067
0.177
9.375 pg/ml 0.125 0.134 0.033
0.143
0 pg/ml 0.098 0.101 0
0.105

Precision

We measured random samples of BPE009 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 31.9 1.2 3.8
Intra-assay 2 20 72.4 2 2.8
Intra-assay 3 20 329 8.2 2.5
Inter-assay 1 20 32.3 2.6 8
Inter-assay 2 20 73.1 5.1 7
Inter-assay 3 20 331 23.8 7.2

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 5*96T

    BPE009

    $2040.00

  • 96T

    BPE009

    $480.00

  • 15*96T

    BPE009

    $5400.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Primacu catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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