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Home > Product > ELISA Primacu > Mouse Cd5 Antigen-Like, Cd5l Primacu™ ELISA Kit

Mouse Cd5 Antigen-Like, Cd5l Primacu™ ELISA Kit

Mouse Cd5 Antigen-Like, Cd5l Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 1.22 pg/mL

Detection range: 6.25-400 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 1.22 pg/mL

Detection range: 6.25-400 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

CD5 antigen-like

Code

BPE281

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

1.22 pg/mL

Detection range

6.25-400 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8°C

Assay time

3.5h

Background

CD5L, also known as CD5 antigen-like, is a soluble protein belonging to group B of the scavenger receptor cysteine-rich (SRCR) superfamily and contains three SRCR domains. It is a secreted glycoprotein and expressed by macrophages presentin lymphoid tissues (spleen, lymph node, thymus, and bone marrow). It binds to myelomonocytic and lymphoid cells and may play an important role in the regulation of the innate and adaptive immune systems. CD5L functions as a pattern recognition molecule by binding both lipoteichoic acid (LTA) on Gram positive and lipopolysaccharide (LPS) on Gram negative bacteria. and the SRCR domain 1 of CD5L retains both the LPS and LTA binding activities. In addtion, it is revealed that CD5L seems to play a role as an inhibitor of apoptosis.

UniProt accession

MASS(Da)

38863

GeneID

11801

Synonyms

Aim;Api6

Gene names

Cd5l

Research area

Cell biology

Target protein

CD5L

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE281 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
400 pg/ml 1.464 1.525 1.415
1.586
200 pg/ml 0.987 0.923 0.813
0.859
100 pg/ml 0.511 0.535 0.425
0.559
50 pg/ml 0.305 0.325 0.215
0.345
25 pg/ml 0.209 0.197 0.087
0.185
12.5 pg/ml 0.144 0.153 0.043
0.162
6.25 pg/ml 0.139 0.133 0.023
0.127
0 pg/ml 0.11 0.11 0
0.109

Precision

We measured random samples of BPE281 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 29.6 1.5 5.1
Intra-assay 2 20 82.1 2.8 3.4
Intra-assay 3 20 293 7.5 2.5
Inter-assay 1 20 29.7 2.6 8.8
Inter-assay 2 20 81.7 8.3 10.2
Inter-assay 3 20 290 26.9 9.3

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 5*96T

    BPE281

    $2320.00

  • 96T

    BPE281

    $580.00

  • 15*96T

    BPE281

    $6525.00

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Lead time: Within two weeks

We are continuously updating our ELISA Primacu catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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