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Home > Product > ELISA Primacu > Mouse Interleukin-6 Receptor Subunit Alpha, Il6ra Primacu™ ELISA Kit

Mouse Interleukin-6 Receptor Subunit Alpha, Il6ra Primacu™ ELISA Kit

Mouse Interleukin-6 Receptor Subunit Alpha, Il6ra Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 1.15 pg/mL

Detection range: 6.25-400 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 1.15 pg/mL

Detection range: 6.25-400 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Interleukin-6 receptor subunit alpha

Code

BPE306

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

1.15 pg/mL

Detection range

6.25-400 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8°C

Assay time

3.5h

Background

Interleukin 6 receptor (IL-6R) also known as CD126 (Cluster of Differentiation 126) is a type I cytokine receptor. The low concentration of a soluble form of IL-6 receptor (SIL-6R) acts as an agonist of IL-6 activity. In the IL-6R/CD126/IL6R system, both a membrane-bound IL-6R and a SIL-6R protein can mediate IL-6 signals into the cells through the interaction of gp130. The resulting IL-6/sIL-6R protein complex is also capable of binding to gp130 and inducing intracellular signaling. Through this so-called 'trans-signaling' mechanism, IL-6 can stimulate cells that lack an endogenous mIL-6R. High levels of IL-6 and SIL-6R have been reported in several chronic inflammatory and autoimmune diseases as well as in cancer. Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy

UniProt accession

MASS(Da)

50455

GeneID

16194

Synonyms

Il6r

Gene names

Il6ra

Research area

Neuroscience,Immunology,Stem cells

Target protein

IL-6R

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE306 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
400 pg/ml 2.833 2.737 2.618
2.641
200 pg/ml 1.535 1.577 1.458
1.619
100 pg/ml 0.753 0.758 0.639
0.763
50 pg/ml 0.445 0.417 0.298
0.39
25 pg/ml 0.303 0.284 0.165
0.266
12.5 pg/ml 0.207 0.2 0.081
0.194
6.25 pg/ml 0.169 0.159 0.04
0.149
0 pg/ml 0.114 0.119 0
0.125

Precision

We measured random samples of BPE306 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 18 1.1 5.9
Intra-assay 2 20 48.7 2.8 5.8
Intra-assay 3 20 222 15.5 7
Inter-assay 1 20 18.1 1.4 7.8
Inter-assay 2 20 48.5 4.8 10
Inter-assay 3 20 225 14.6 6.5

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 5*96T

    BPE306

    $2040.00

  • 96T

    BPE306

    $480.00

  • 15*96T

    BPE306

    $5400.00

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Lead time: Within two weeks

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