Human Interleukin-13, IL13 Primacu™ ELISA Kit

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OneStep ELISA kit
Primacu ELISA kit
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Protéines recombinantes

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Coagulation sanguine
Métabolisme
Cancer
Microbiologie
Cardiovasculaire
Neurosciences
Biologie cellulaire
Transduction du signal
Biologie du développement
Cellules souches
Epigénétique et signalisation nucléaire
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Home > Product > ELISA Primacu > Human Interleukin-13, IL13 Primacu™ ELISA Kit

Human Interleukin-13, IL13 Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 1.68 pg/mL

Detection range: 4.69-300 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 1.68 pg/mL

Detection range: 4.69-300 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Interleukin-13

Code

BPE074

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

1.68 pg/mL

Detection range

4.69-300 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

Interleukin 13 (IL-13) is a single-chain glycosylated polypeptide, which belongs to the IL-13/IL-4 family. IL-13 protein is secreted by many cell types, but especially by T helper type 2 (Th2) cells. IL-13 exerts its effects through a multi-subunit receptor comprising the alpha chain of the IL-4 receptor (IL-4Rα) and at least one of two known IL-13-specific binding chains (IL-13 Rα1 and IL-13 Rα2). As a cytokine, IL-13 protein is critical in regulating inflammatory, immune responses, and diseases. Also, it inhibits the production of pro-inflammatory cytokines and chemokines, and thus down-regulates macrophage activity. IL-13 protein and antibody are more importantly implicated as a central mediator of immunoregulatory processes in various cell types. Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy

UniProt accession

P35225

MASS（Da）

15816

GeneID

3596

Synonyms

NC30

Gene names

IL13

Research area

Cancer,Immunology,Cardiovascular,Neuroscience

Target protein

IL-13

Components

Components	Quantity
Pre-coated ELISA Plate	12 × 8 well strips × 1
Standard Solution	0.2mL × 1 vail
Dilution Buffer	20mL × 1 vail
Stop Solution	6mL × 1 vail
Substrate Solution A	12mL × 1 vail
Substrate Solution B	12mL × 1 vail
Wash Buffer Concentrate (25x)	20mL × 1 vail
Detection Antibody Concentrate	0.6mL × 1 vail

Protocol data

These standard curves of BPE074 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
300 pg/ml	2.599	2.558	2.437
	2.516
150 pg/ml	1.412	1.399	1.278
	1.386
75 pg/ml	0.763	0.791	0.67
	0.818
37.5 pg/ml	0.406	0.435	0.314
	0.464
18.75 pg/ml	0.286	0.274	0.153
	0.261
9.375 pg/ml	0.199	0.197	0.076
	0.194
4.6875 pg/ml	0.151	0.155	0.034
	0.159
0 pg/ml	0.124	0.121	0
	0.117

Precision

We measured random samples of BPE074 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	20	25	2.3	9.2
Intra-assay	2	20	51.1	4.7	9.3
Intra-assay	3	20	222	10.7	4.8
Inter-assay	1	20	25	2.4	9.6
Inter-assay	2	20	51.2	4.4	8.6
Inter-assay	3	20	224	16.4	7.3

Protocols

> Protocol Booklet

References

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Associated products

Datasheet

Size

Code

Price

5*96T

BPE074

$2040.00
96T

BPE074

$480.00
15*96T

BPE074

$5400.00

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Lead time: Within two weeks

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