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Home > Product > Biochemistry > Reactive Oxygen Species, ROS Assay Kit

Reactive Oxygen Species, ROS Assay Kit

Reactive Oxygen Species, ROS Assay Kit

Product Summary

Size: 100T

Code: SH0403

Detection method: Chemical fluorometric method

Assay type: Cell-based (quantitative)

Sample type: Fresh tissue, cultured cells

Product Summary

Size: 100T

Code: SH0403

Detection method: Chemical fluorometric method

Assay type: Cell-based (quantitative)

Sample type: Fresh tissue, cultured cells

Product overview

Product name

Reactive Oxygen Species, ROS Assay Kit

Background

Reactive oxygen species (ROS) include the super oxygen free radical, hydrogen peroxide and its downstream product (peroxide and hydroxide) etc. ROS is involved in cell growth, proliferation, differentiation of development, aging and apoptosis as well as many physiological and disease process. The DCFH-DA (2, 7-dichlorofuorescin diacetate) is the most commonly used and most sensitive intracellular reactive oxygen detection probe. DCFH-DA has no fluorescence, it is hydrolyzed into DCFH (dichlorofluorescin) by ester hydrolysis enzyme after entering cells. DCFH can be oxidized to strong green fluorescence material DCF (dichlorofluorescein) that cannot pass through cell membrane when ROS exists. The excitation wavelength and the maximum emission wavelength of DCF is 502nm and 530nm respectively. The fluorescence intensity is proportional to the level of reactive oxygen species in cells.

Storage

2-8ºC

Target protein

Reactive oxygen

Components

Components Quantity
DCFH-DA in DMSO, 10 mM 0.1 ml
ROS hydrogen donor 1 ml

Protocols

References

Euxanthone improves cognitive impairment by attenuating mitochondrial fragmentation and suppressing oxidative stress

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  • Size

    Code

    Price

  • 100T

    SH0403

    $268.00

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Lead time: Within two weeks

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