Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Porcine Ubiquitin Carboxyl-terminalhydrolase Isozyme L1, UCHL1 ELISA Kit

Porcine Ubiquitin Carboxyl-terminalhydrolase Isozyme L1, UCHL1 ELISA Kit

Porcine Ubiquitin Carboxyl-terminalhydrolase Isozyme L1, UCHL1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 2.21ng/L

Detection range: 5-1000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Porcine

Product Summary

Size: 48T,96T

Sensitivity: 2.21ng/L

Detection range: 5-1000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Porcine

Product overview

Full product name

Ubiquitin carboxyl-terminal hydrolase isozyme L1

Code

E0611Po

Assay type

Sandwich

Size

48T,96T

Sensitivity

2.21ng/L

Detection range

5-1000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Porcine

Storage

2-8ºC

Assay time

1h 30m

Background

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin (By similarity). Also binds to free monoubiquitin and may prevent its degradation in lysosomes (By similarity). The homodimer may have ATP-independent ubiquitin ligase activity (By similarity).

UniProt accession

MASS(Da)

24,859

GeneID

396637

Synonyms

Ubiquitin carboxyl-terminal hydrolase isozyme L1;Ubiquitin thioesterase L1;UCHL1;UCH-L1

Gene names

UCHL1

Research area

Neuroscience; Cell Biology; Tags & Cell Markers

Target protein

UCHL1

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0611Po are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
640ng/L 2.037 2.131 2.089
2.226
320ng/L 1.801 1.774 1.731
1.746
160ng/L 1.276 1.232 1.19
1.188
80ng/L 0.622 0.618 0.575
0.613
40ng/L 0.398 0.402 0.36
0.406
0ng/L 0.032 0.042 0
0.053

Precision

We measured random samples of E0611Po within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 114.64 6.6 5.76
Intra-Assay 2 18 186.32 6.61 3.55
Intra-Assay 3 18 49.66 2.95 5.94

Protocols

References

Customer reviews and Q&As

No reviews
No reviews
  • Size

    Code

    Price

  • 48T

    E0611Po

    $320.00

  • 96T

    E0611Po

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up