Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E0623Po are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1200ng/L | 1.918 | 1.862 | 1.819 |
| 1.806 | |||
| 600ng/L | 0.924 | 0.997 | 0.955 |
| 1.071 | |||
| 300ng/L | 0.545 | 0.581 | 0.538 |
| 0.616 | |||
| 150ng/L | 0.226 | 0.213 | 0.17 |
| 0.2 | |||
| 75ng/L | 0.109 | 0.111 | 0.069 |
| 0.114 | |||
| 0ng/L | 0.048 | 0.043 | 0 |
| 0.038 |
Precision
We measured random samples of E0623Po within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 1121.82 | 41.33 | 3.68 |
| Intra-Assay | 2 | 18 | 723.53 | 36.39 | 5.03 |
| Intra-Assay | 3 | 18 | 253.95 | 9.23 | 3.63 |
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