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Home > Product > ELISA Kit > Human Forkhead Box O4, FOXO4 ELISA Kit

Human Forkhead Box O4, FOXO4 ELISA Kit

Human Forkhead Box O4, FOXO4 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 8.84ng/L

Detection range: 20-3500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 8.84ng/L

Detection range: 20-3500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

forkhead box O4

Code

E0629Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

8.84ng/L

Detection range

20-3500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Involved in increased proteasome activity in embryonic stem cells (ESCs) by activating expression of PSMD11 in ESCs, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity.

UniProt accession

MASS(Da)

53,684

GeneID

4303

Synonyms

AFX;AFX1;Fork head domain transcription factor AFX1;Forkhead box protein O4;FOXO4;MLLT7

Gene names

FOXO4

Research area

Epigenetics and Nuclear Signaling

Target protein

FOXO4

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0629Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2000ng/L 2.118 2.102 2.05
2.086
1000ng/L 1.445 1.402 1.35
1.359
500ng/L 0.588 0.633 0.58
0.678
250ng/L 0.364 0.377 0.325
0.391
125ng/L 0.142 0.138 0.086
0.134
0ng/L 0.055 0.052 0
0.05

Precision

We measured random samples of E0629Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 798.38 33.61 4.21
Intra-Assay 2 18 1159.8 30.41 2.62
Intra-Assay 3 18 483.02 14.51 3

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E0629Hu

    $320.00

  • 96T

    E0629Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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