Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E0643Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 20ng/mL | 2.022 | 2.053 | 1.997 |
| 2.083 | |||
| 10ng/mL | 0.924 | 1.028 | 0.972 |
| 1.131 | |||
| 5ng/mL | 0.532 | 0.543 | 0.487 |
| 0.554 | |||
| 2.5ng/mL | 0.283 | 0.262 | 0.206 |
| 0.242 | |||
| 1.25ng/mL | 0.143 | 0.15 | 0.094 |
| 0.156 | |||
| 0ng/mL | 0.061 | 0.056 | 0 |
| 0.051 |
Precision
We measured random samples of E0643Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 15.11 | 0.42 | 2.76 |
| Intra-Assay | 2 | 18 | 6.76 | 0.35 | 5.21 |
| Intra-Assay | 3 | 18 | 9.66 | 0.47 | 4.91 |
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