Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS（Da）
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E0699Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 16ng/ml | 2.293 | 2.347 | 2.315 |
| 2.401 | |||
| 8ng/ml | 1.318 | 1.277 | 1.245 |
| 1.236 | |||
| 4ng/ml | 0.578 | 0.638 | 0.606 |
| 0.698 | |||
| 2ng/ml | 0.387 | 0.355 | 0.323 |
| 0.323 | |||
| 1ng/ml | 0.119 | 0.14 | 0.108 |
| 0.161 | |||
| 0ng/ml | 0.042 | 0.032 | 0 |
| 0.022 |
Precision
We measured random samples of E0699Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 11.75 | 0.34 | 2.85 |
| Intra-Assay | 2 | 18 | 2.99 | 0.16 | 5.33 |
| Intra-Assay | 3 | 18 | 7.31 | 0.39 | 5.37 |
Protocols
References (1)
Customer reviews and Q&As
