Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS（Da）
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E0769Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 24ng/ml | 1.858 | 1.831 | 1.788 |
| 1.804 | |||
| 12ng/ml | 0.992 | 0.943 | 0.9 |
| 0.893 | |||
| 6ng/ml | 0.514 | 0.501 | 0.458 |
| 0.487 | |||
| 3ng/ml | 0.297 | 0.221 | 0.179 |
| 0.146 | |||
| 1.5ng/ml | 0.149 | 0.14 | 0.097 |
| 0.13 | |||
| 0ng/ml | 0.042 | 0.043 | 0 |
| 0.044 |
Precision
We measured random samples of E0769Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 9.13 | 0.47 | 5.1 |
| Intra-Assay | 2 | 18 | 23.96 | 0.79 | 3.31 |
| Intra-Assay | 3 | 18 | 14.15 | 0.68 | 4.78 |
Protocols
References
Customer reviews and Q&As
