Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E0806Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 80ng/ml | 2.141 | 2.258 | 2.212 |
| 2.375 | |||
| 40ng/ml | 1.673 | 1.606 | 1.56 |
| 1.539 | |||
| 20ng/ml | 1.076 | 1.03 | 0.984 |
| 0.984 | |||
| 10ng/ml | 0.702 | 0.675 | 0.629 |
| 0.648 | |||
| 5ng/ml | 0.431 | 0.42 | 0.374 |
| 0.409 | |||
| 0ng/ml | 0.061 | 0.046 | 0 |
| 0.031 |
Precision
We measured random samples of E0806Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 25.27 | 1.75 | 6.91 |
| Intra-Assay | 2 | 18 | 38.98 | 1.64 | 4.2 |
| Intra-Assay | 3 | 18 | 10.79 | 0.75 | 6.97 |
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