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Home > Product > ELISA Kit > Rat Endothelin 2, ET-2 ELISA Kit

Rat Endothelin 2, ET-2 ELISA Kit

Rat Endothelin 2, ET-2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.51ng/L

Detection range: 1-300ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.51ng/L

Detection range: 1-300ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Endothelin 2

Code

E1006Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.51ng/L

Detection range

1-300ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Endothelin 2 (ET-2) is a protein encoded by the EDN2 gene in humans. It was first discovered in 1988 by Yanagisawa and team and belongs to a family of three endothelin peptide isoforms (ET-1, ET-2, ET-3), which constrict blood vessels. ET-2 is encoded by genes on separate chromosomes to its isoforms and is mainly produced in vascular endothelial cells of the kidney, placenta, uterus, heart, central nervous system and intestine. It becomes present in the blood of animals and humans at levels ranging from 0.3pg/ml to 3pg/ml. ET-2 acts by binding to two different G-protein coupled receptors (GCPRs), the endothelin A receptor (EDNRA) and the endothelin B receptor (EDNRB).

UniProt accession

MASS(Da)

19,478

GeneID

24324

Synonyms

Endothelin-2;Et2;ET-2;PPET2;Preproendothelin-2;RNEDN2S01;Vasoactive intestinal contractor;VIC

Gene names

Edn2

Research area

Epigenetics and Nuclear Signaling

Target protein

ET-2

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of Rat Endothelin 2, ET-2 ELISA Kit are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
160ng/L 2.372 2.391 2.352
2.411
80ng/L 1.23 1.32 1.281
1.411
40ng/L 0.636 0.709 0.67
0.783
20ng/L 0.357 0.373 0.334
0.389
10ng/L 0.191 0.172 0.133
0.153
0ng/L 0.043 0.039 0
0.036

Precision

We measured random samples of E1006Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 77.63 2.53 3.26
Intra-Assay 2 18 33.57 1.62 4.81
Intra-Assay 3 18 45.1 1.86 4.12

Protocols

References

Customer reviews and Q&As

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Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

Add to Cart

Lead time: Within one week

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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