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Home > Product > ELISA Kit > Human Kremen Protein 1, KREMEN1 ELISA Kit

Human Kremen Protein 1, KREMEN1 ELISA Kit

Human Kremen Protein 1, KREMEN1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.98ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.98ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Kremen protein 1

Code

E1160Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.98ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Receptor for Dickkopf proteins. Cooperates with DKK1/2 to inhibit Wnt/beta-catenin signaling by promoting the endocytosis of Wnt receptors LRP5 and LRP6. In the absence of DKK1, potentiates Wnt-beta-catenin signaling by maintaining LRP5 or LRP6 at the cell membrane. Can trigger apoptosis in a Wnt-independent manner and this apoptotic activity is inhibited upon binding of the ligand DKK1. Plays a role in limb development; attenuates Wnt signaling in the developing limb to allow normal limb patterning and can also negatively regulate bone formation. Modulates cell fate decisions in the developing cochlea with an inhibitory role in hair cell fate specification.

UniProt accession

MASS(Da)

51,744

GeneID

83999

Synonyms

Dickkopf receptor;ECTD13;KREMEN;Kremen protein 1;KREMEN1;Kringle domain-containing transmembrane protein 1;Kringle-containing protein marking the eye and the nose;KRM1

Gene names

KREMEN1

Research area

Signal Transduction

Target protein

KREMEN1

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1160Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.195 2.192 2.153
2.189
1200ng/L 1.187 1.262 1.223
1.338
600ng/L 0.668 0.675 0.636
0.682
300ng/L 0.411 0.399 0.36
0.388
150ng/L 0.154 0.176 0.137
0.198
0ng/L 0.032 0.039 0
0.046

Precision

We measured random samples of E1160Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1281.65 41.91 3.27
Intra-Assay 2 18 690.91 39.72 5.75
Intra-Assay 3 18 581.05 40.61 6.99

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E1160Hu

    $320.00

  • 96T

    E1160Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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