Full product name

Code

Assay type

Size

Sensitivity

Detection range

Sample type

Species

Storage

Assay time

UniProt accession

MASS（Da）

GeneID

Synonyms

Gene names

Research area

Target protein

Components	Quantity
Pre-coated ELISA plate	12 wells * 8 detachable strips
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

These standard curves of E1253Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
20ng/mL	1.949	1.852	1.793
	1.756
10ng/mL	1.037	0.996	0.936
	0.954
5ng/mL	0.461	0.491	0.431
	0.52
2.5ng/mL	0.253	0.261	0.201
	0.268
1.25ng/mL	0.145	0.114	0.054
	0.082
0ng/mL	0.056	0.06	0
	0.063

We measured random samples of E1253Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	15.35	0.36	2.33
Intra-Assay	2	18	9.66	0.36	3.78
Intra-Assay	3	18	7	0.35	4.96

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