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Home > Product > ELISA Kit > Mouse Fibronectin Type 3 Domain-containing Protein 5, FNDC5 ELISA Kit

Mouse Fibronectin Type 3 Domain-containing Protein 5, FNDC5 ELISA Kit

Mouse Fibronectin Type 3 Domain-containing Protein 5, FNDC5 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 1.04ng/L

Detection range: 2-700ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 1.04ng/L

Detection range: 2-700ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Fibronectin type 3 domain-containing protein 5

Code

E1278Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

1.04ng/L

Detection range

2-700ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Fibronectin type III domain-containing protein 5, the precursor of irisin, is a type I transmembrane glycoprotein that is encoded by the FNDC5 gene. Irisin is a cleaved version of FNDC5, named after the Greek messenger goddess Iris. Fibronectin domain-containing protein 5 is a membrane protein comprising a short cytoplasmic domain, a transmembrane segment, and an ectodomain consisting of a ~100 kDa fibronectin type III (FNIII) domain.

UniProt accession

MASS(Da)

23,321

GeneID

384061

Synonyms

1500001L03Rik;AI836596;C87088;Fibronectin type III domain-containing protein 5;Fibronectin type III repeat-containing protein 2;FNDC5;Irisin];PeP;Peroxisomal protein;Pxp

Gene names

Fndc5

Research area

Others

Target protein

FNDC5

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1278Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
400ng/L 2.313 2.366 2.298
2.42
200ng/L 1.294 1.368 1.3
1.443
100ng/L 0.762 0.75 0.682
0.737
50ng/L 0.401 0.477 0.409
0.554
25ng/L 0.243 0.264 0.196
0.285
0ng/L 0.077 0.068 0
0.059

Precision

We measured random samples of E1278Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 141.18 7.22 5.12
Intra-Assay 2 18 291.75 7.74 2.65
Intra-Assay 3 18 47.4 2.62 5.52

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E1278Mo

    $320.00

  • 96T

    E1278Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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