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Home > Product > ELISA Kit > Rat Toll Interacting Protein, TOLLIP ELISA Kit

Rat Toll Interacting Protein, TOLLIP ELISA Kit

Rat Toll Interacting Protein, TOLLIP ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.028ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.028ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Toll Interacting Protein

Code

E1321Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.028ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Component of the signaling pathway of IL-1 and Toll-like receptors. Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex. Inhibits IRAK1 phosphorylation and kinase activity. Connects the ubiquitin pathway to autophagy by functioning as a ubiquitin-ATG8 family adapter and thus mediating autophagic clearance of ubiquitin conjugates. The TOLLIP-dependent selective autophagy pathway plays an important role in clearance of cytotoxic polyQ proteins aggregates (By similarity).

UniProt accession

MASS(Da)

30,315

GeneID

361677

Synonyms

Toll-interacting protein;TOLLIP

Gene names

Tollip

Research area

Others

Target protein

TOLLIP

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1321Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/ml 2.151 2.174 2.133
2.198
6ng/ml 1.396 1.423 1.381
1.45
3ng/ml 0.703 0.709 0.668
0.715
1.5ng/ml 0.329 0.332 0.291
0.336
0.75ng/ml 0.208 0.199 0.157
0.189
0ng/ml 0.032 0.042 0
0.051

Precision

We measured random samples of E1321Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 3.74 0.17 4.68
Intra-Assay 2 18 7.4 0.27 3.71
Intra-Assay 3 18 1.54 0.06 3.73

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E1321Ra

    $320.00

  • 96T

    E1321Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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