Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS（Da）
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E1396Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 80ng/ml | 2.013 | 1.997 | 1.957 |
| 1.982 | |||
| 40ng/ml | 1.496 | 1.477 | 1.436 |
| 1.458 | |||
| 20ng/ml | 0.627 | 0.595 | 0.555 |
| 0.563 | |||
| 10ng/ml | 0.365 | 0.334 | 0.293 |
| 0.302 | |||
| 5ng/ml | 0.201 | 0.19 | 0.149 |
| 0.178 | |||
| 0ng/ml | 0.023 | 0.04 | 0 |
| 0.058 |
Precision
We measured random samples of E1396Ra within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 26.17 | 0.97 | 3.7 |
| Intra-Assay | 2 | 18 | 34.21 | 1.05 | 3.08 |
| Intra-Assay | 3 | 18 | 19.5 | 0.68 | 3.47 |
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