3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1

E1432Ra

Sandwich

48T,96T

0.021ng/ml

0.05-20ng/ml

Serum, plasma, cell culture supernates

Rat

2-8ºC

1h 30m

A bifunctional enzyme responsible for the oxidation and isomerization of 3beta-hydroxy-Delta5-steroid precursors to 3-oxo-Delta4-steroids, an essential step in steroid hormone biosynthesis. Specifically catalyzes the conversion of pregnenolone to progesterone, dehydroepiandrosterone (DHEA) to 4-androstenedione, and androstenediol to testosterone (PubMed:1537836, PubMed:1985917). Additionally, catalyzes the interconversion between 3beta-hydroxy and 3-oxo-5alpha-androstane steroids controlling the bioavalability of the active forms. Specifically converts dihydrotestosterone to its inactive form 5alpha-androstanediol, that does not bind androgen receptor/AR. Also converts androstanedione, a precursor of testosterone and estrone, to epiandrosterone (PubMed:1537836). Expected to use NAD+ as preferred electron donor for the 3beta-hydroxy-steroid dehydrogenase activity and NADPH for the 3-ketosteroid reductase activity (Probable).

42,037

360348

3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1;3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I;3-beta-HSD I;3-beta-hydroxy-5-ene steroid dehydrogenase;3-beta-hydroxy-Delta(5-steroid dehydrogenase;3-beta-hydroxysteroid 3-dehydrogenase;Delta-5-3-ketosteroid isomerase;Dihydrotestosterone oxidoreductase;HSD3B1;Steroid Delta-isomerase

Hsd3b1

Signal Transduction; Cancer; Metabolism

HSD3B1