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Home > Product > ELISA Kit > Rat Protein Disulfide-isomerase A3, PDIA3 ELISA Kit

Rat Protein Disulfide-isomerase A3, PDIA3 ELISA Kit

Rat Protein Disulfide-isomerase A3, PDIA3 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.27ng/ml

Detection range: 0.5-150ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.27ng/ml

Detection range: 0.5-150ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Protein disulfide-isomerase A3

Code

E1505Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.27ng/ml

Detection range

0.5-150ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Protein disulfide-isomerase A3 (PDIA3), also known as glucose-regulated protein, 58-kD (GRP58), is an isomerase enzyme.[5][6][7] This protein localizes to the endoplasmic reticulum (ER) and interacts with lectin chaperones calreticulin and calnexin (CNX) to modulate folding of newly synthesized glycoproteins. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.

UniProt accession

MASS(Da)

56,623

GeneID

29468

Synonyms

58 kDa glucose-regulated protein;58 kDa microsomal protein;Disulfide isomerase ER-60;Endoplasmic reticulum resident protein 57;Endoplasmic reticulum resident protein 60;ER protein 57;ER protein 60;ER60;ERp57;ERp60;Grp58;HIP-70;p58;PDIA3;Protein disulfide-isomerase A3;Q-2

Gene names

Pdia3

Research area

Others

Target protein

PDIA3

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1505Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
80ng/ml 2.244 2.277 2.212
2.309
40ng/ml 1.321 1.297 1.232
1.273
20ng/ml 0.763 0.725 0.66
0.688
10ng/ml 0.438 0.497 0.432
0.556
5ng/ml 0.28 0.305 0.24
0.33
0ng/ml 0.07 0.065 0
0.061

Precision

We measured random samples of E1505Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 34.66 2.04 5.89
Intra-Assay 2 18 40.41 1.25 3.1
Intra-Assay 3 18 19.81 1.3 6.56

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E1505Ra

    $320.00

  • 96T

    E1505Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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