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Home > Product > ELISA Kit > Mouse Nad Kinase, NADK ELISA Kit

Mouse Nad Kinase, NADK ELISA Kit

Mouse Nad Kinase, NADK ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.28ng/ml

Detection range: 0.5-100ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.28ng/ml

Detection range: 0.5-100ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

NAD Kinase

Code

E1540Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.28ng/ml

Detection range

0.5-100ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

NAD+ kinase (EC 2.7.1.23, NADK) is an enzyme that converts nicotinamide adenine dinucleotide (NAD+) into NADP+ through phosphorylating the NAD+ coenzyme. NADP+ is an essential coenzyme that is reduced to NADPH primarily by the pentose phosphate pathway to provide reducing power in biosynthetic processes such as fatty acid biosynthesis and nucleotide synthesis. The structure of the NADK from the archaean Archaeoglobus fulgidus has been determined. In humans, the genes NADK and MNADK encode NAD+ kinases localized in cytosol and mitochondria, respectively. Similarly, yeast have both cytosolic and mitochondrial isoforms, and the yeast mitochondrial isoform accepts both NAD+ and NADH as substrates for phosphorylation.

UniProt accession

MASS(Da)

48,597

GeneID

192185

Synonyms

4432404C02Rik;BC004012;NAD kinase;NADK;Poly(P/ATP NAD kinase

Gene names

Nadk

Research area

Others

Target protein

NADK

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1540Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
72ng/ml 1.893 1.989 1.954
2.086
36ng/ml 1.136 1.047 1.012
0.957
18ng/ml 0.405 0.496 0.461
0.587
9ng/ml 0.162 0.239 0.204
0.316
4.5ng/ml 0.104 0.117 0.082
0.131
0ng/ml 0.039 0.035 0
0.032

Precision

We measured random samples of E1540Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 37.02 1.82 4.91
Intra-Assay 2 18 49.91 1.37 2.74
Intra-Assay 3 18 19.37 1.03 5.34

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E1540Mo

    $320.00

  • 96T

    E1540Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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