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Home > Product > ELISA Kit > Mouse Acylamino-acid-releasing Enzyme, APEH ELISA Kit

Mouse Acylamino-acid-releasing Enzyme, APEH ELISA Kit

Mouse Acylamino-acid-releasing Enzyme, APEH ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.043ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.043ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Acylamino-acid-releasing enzyme

Code

E1553Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.043ng/ml

Detection range

0.1-40ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

This enzyme catalyzes the hydrolysis of the N-terminal peptide bond of an N-acetylated peptide to generate an N-acetylated amino acid and a peptide with a free N-terminus. It preferentially cleaves off Ac-Ala, Ac-Met and Ac-Ser (By similarity).

UniProt accession

MASS(Da)

81,581

GeneID

235606

Synonyms

AARE;Acylamino-acid-releasing enzyme;Acylaminoacyl-peptidase;Acyl-peptide hydrolase;APEH;APH

Gene names

Apeh

Research area

Cancer; Cell Biology

Target protein

APEH

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of Mouse Acylamino-acid-releasing Enzyme, APEH ELISA Kit are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/ml 1.706 1.83 1.781
1.953
12ng/ml 0.923 0.974 0.924
1.025
6ng/ml 0.555 0.536 0.487
0.517
3ng/ml 0.242 0.196 0.146
0.149
1.5ng/ml 0.099 0.124 0.074
0.149
0ng/ml 0.051 0.049 0
0.048

Precision

We measured random samples of E1553Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 14.72 0.68 4.61
Intra-Assay 2 18 17.92 0.5 2.82
Intra-Assay 3 18 6.73 0.35 5.22

Protocols

References

Customer reviews and Q&As

No reviews
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Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

Add to Cart

Lead time: Within one week

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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