Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E1573Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1200ng/L | 2.26 | 2.245 | 2.196 |
| 2.231 | |||
| 600ng/L | 1.288 | 1.263 | 1.214 |
| 1.238 | |||
| 300ng/L | 0.752 | 0.701 | 0.652 |
| 0.65 | |||
| 150ng/L | 0.534 | 0.458 | 0.409 |
| 0.381 | |||
| 75ng/L | 0.241 | 0.227 | 0.177 |
| 0.212 | |||
| 0ng/L | 0.042 | 0.049 | 0 |
| 0.057 |
Precision
We measured random samples of E1573Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 473.85 | 23.86 | 5.03 |
| Intra-Assay | 2 | 18 | 789.27 | 21.64 | 2.74 |
| Intra-Assay | 3 | 18 | 191.71 | 13.98 | 7.29 |
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