Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E1594Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 8ng/ml | 1.832 | 1.757 | 1.714 |
| 1.682 | |||
| 4ng/ml | 0.942 | 0.982 | 0.939 |
| 1.021 | |||
| 2ng/ml | 0.521 | 0.509 | 0.466 |
| 0.496 | |||
| 1ng/ml | 0.186 | 0.222 | 0.179 |
| 0.258 | |||
| 0.5ng/ml | 0.079 | 0.086 | 0.043 |
| 0.094 | |||
| 0ng/ml | 0.051 | 0.043 | 0 |
| 0.035 |
Precision
We measured random samples of E1594Mo within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 2.41 | 0.11 | 4.54 |
| Intra-Assay | 2 | 18 | 4.11 | 0.2 | 4.9 |
| Intra-Assay | 3 | 18 | 8.5 | 0.4 | 4.67 |
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