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Home > Product > ELISA Kit > Mouse Aryl Hydrocarbon Receptor Nuclear Translocator, ARNT ELISA Kit

Mouse Aryl Hydrocarbon Receptor Nuclear Translocator, ARNT ELISA Kit

Mouse Aryl Hydrocarbon Receptor Nuclear Translocator, ARNT ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.89ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 7.89ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Aryl Hydrocarbon Receptor Nuclear Translocator

Code

E1611Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.89ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens (By similarity). The heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters and functions as a transcriptional regulator of the adaptive response to hypoxia (PubMed:26245371, PubMed:27782878). The heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (PubMed:28602820).

UniProt accession

MASS(Da)

86,963

GeneID

11863

Synonyms

ARNT;ARNT protein;Aryl hydrocarbon receptor nuclear translocator;bHLHe;bHLHe2;D3Ertd557;D3Ertd557e;Dioxin receptor, nuclear translocator;Drnt;ESTM4;ESTM42;Hif1;Hif1b;HIF1-beta;HIF-1-beta;Hypoxia-inducible factor 1-beta;mKIAA4051

Gene names

Arnt

Research area

Neuroscience

Target protein

ARNT

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1611Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 1.956 2.055 2.021
2.154
800ng/L 1.341 1.374 1.34
1.407
400ng/L 0.749 0.657 0.622
0.565
200ng/L 0.418 0.436 0.402
0.454
100ng/L 0.118 0.165 0.131
0.212
0ng/L 0.026 0.034 0
0.043

Precision

We measured random samples of E1611Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 987.93 39.54 4
Intra-Assay 2 18 467.81 19.99 4.27
Intra-Assay 3 18 311.1 15.93 5.12

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E1611Mo

    $320.00

  • 96T

    E1611Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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