Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E1613Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 40ng/ml | 2.441 | 2.482 | 2.427 |
| 2.523 | |||
| 20ng/ml | 1.448 | 1.426 | 1.371 |
| 1.405 | |||
| 10ng/ml | 0.846 | 0.843 | 0.789 |
| 0.841 | |||
| 5ng/ml | 0.393 | 0.418 | 0.363 |
| 0.443 | |||
| 2.5ng/ml | 0.235 | 0.257 | 0.202 |
| 0.279 | |||
| 0ng/ml | 0.044 | 0.055 | 0 |
| 0.066 |
Precision
We measured random samples of E1613Mo within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 22.73 | 0.7 | 3.1 |
| Intra-Assay | 2 | 18 | 8.76 | 0.46 | 5.31 |
| Intra-Assay | 3 | 18 | 11.18 | 0.59 | 5.26 |
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