Protein-arginine deiminase type-4

E1640Mo

Sandwich

48T,96T

0.08ng/ml

0.2-60ng/ml

Serum, plasma, cell culture supernates

Mouse

2-8ºC

1h 30m

Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance (PubMed:15339660, PubMed:32528174). Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci) (PubMed:15339660). Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1: citrullination of 'Arg-54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance (PubMed:24463520, PubMed:32528174). Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci (PubMed:20733033, PubMed:23650392). Required for the formation of neutrophil extracellular traps (NETs); NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (PubMed:20733033, PubMed:32528174). Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription (By similarity). Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1 (By similarity).

74,415

18602

Pa;Pad4;PADI4;Pd;Pdi4;Peptidylarginine deiminase IV;Protein-arginine deiminase type IV;Protein-arginine deiminase type-4

Padi4

Signal Transduction

PADI4