Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS（Da）
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E1658Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 800ng/L | 1.926 | 1.815 | 1.771 |
| 1.703 | |||
| 400ng/L | 1.069 | 1.104 | 1.06 |
| 1.139 | |||
| 200ng/L | 0.519 | 0.517 | 0.473 |
| 0.514 | |||
| 100ng/L | 0.162 | 0.173 | 0.129 |
| 0.183 | |||
| 50ng/L | 0.126 | 0.114 | 0.07 |
| 0.102 | |||
| 0ng/L | 0.037 | 0.044 | 0 |
| 0.05 |
Precision
We measured random samples of E1658Mo within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 789.5 | 40.99 | 5.19 |
| Intra-Assay | 2 | 18 | 386.09 | 19.21 | 4.98 |
| Intra-Assay | 3 | 18 | 255.53 | 10.25 | 4.01 |
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