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Home > Product > ELISA Kit > Rat Mothers Against Decapentaplegic Homolog 5, SMAD5 ELISA Kit

Rat Mothers Against Decapentaplegic Homolog 5, SMAD5 ELISA Kit

Rat Mothers Against Decapentaplegic Homolog 5, SMAD5 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.19ng/L

Detection range: 10-3800ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 5.19ng/L

Detection range: 10-3800ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Mothers Against Decapentaplegic Homolog 5

Code

E1690Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.19ng/L

Detection range

10-3800ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD) (By similarity).

UniProt accession

MASS(Da)

52,215

GeneID

59328

Synonyms

MAD homolog 5;Madh5;Mothers against decapentaplegic homolog 5;Mothers against DPP homolog 5;SMAD 5;SMAD family member 5;Smad5

Gene names

Smad5

Research area

Signal Transduction; Epigenetics and Nuclear Signaling; Stem Cells; Developmental Biology

Target protein

Smad5

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1690Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2000ng/L 2.408 2.433 2.38
2.458
1000ng/L 1.461 1.481 1.428
1.5
500ng/L 0.712 0.815 0.762
0.917
250ng/L 0.435 0.474 0.421
0.513
125ng/L 0.212 0.216 0.163
0.22
0ng/L 0.04 0.053 0
0.065

Precision

We measured random samples of E1690Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 282.42 18.03 6.38
Intra-Assay 2 18 783.04 39.84 5.09
Intra-Assay 3 18 1112.49 34.26 3.08

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E1690Ra

    $320.00

  • 96T

    E1690Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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