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Home > Product > ELISA Kit > Rat Gtpase Hras, HRAS ELISA Kit

Rat Gtpase Hras, HRAS ELISA Kit

Rat Gtpase Hras, HRAS ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 3.65ng/L

Detection range: 6-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 3.65ng/L

Detection range: 6-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

GTPase HRas

Code

E1736Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

3.65ng/L

Detection range

6-1500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

GTPase HRas, from "Harvey Rat sarcoma virus", also known as transforming protein p21 is an enzyme that in humans is encoded by the HRAS gene. The HRAS gene is located on the short (p) arm of chromosome 11 at position 15.5, from base pair 522,241 to base pair 525,549. HRas is a small G protein in the Ras subfamily of the Ras superfamily of small GTPases. Once bound to Guanosine triphosphate, H-Ras will activate a Raf kinase like c-Raf, the next step in the MAPK/ERK pathway.

UniProt accession

MASS(Da)

21,298

GeneID

293621

Synonyms

c-H-ras;GTPase HRas;GTPase HRas, N-terminally processed];HRas;HRAS1;H-Ras-1;p21ras;Transforming protein p21

Gene names

Hras

Research area

Cell Biology

Target protein

HRas

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1736Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
800ng/L 1.861 1.95 1.901
2.038
400ng/L 1.124 1.088 1.039
1.051
200ng/L 0.486 0.534 0.486
0.582
100ng/L 0.214 0.237 0.189
0.26
50ng/L 0.136 0.104 0.055
0.072
0ng/L 0.057 0.049 0
0.04

Precision

We measured random samples of E1736Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 702.61 28.1 4
Intra-Assay 2 18 242.7 9.64 3.97
Intra-Assay 3 18 293.2 11.92 4.07

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E1736Ra

    $320.00

  • 96T

    E1736Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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