Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E1754Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 36ng/ml | 2.072 | 2.14 | 2.101 |
| 2.208 | |||
| 18ng/ml | 1.698 | 1.704 | 1.665 |
| 1.71 | |||
| 9ng/ml | 1.114 | 1.133 | 1.094 |
| 1.152 | |||
| 4.5ng/ml | 0.699 | 0.699 | 0.66 |
| 0.699 | |||
| 2.25ng/ml | 0.429 | 0.441 | 0.402 |
| 0.454 | |||
| 0ng/ml | 0.031 | 0.039 | 0 |
| 0.047 |
Precision
We measured random samples of E1754Mo within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 7.08 | 0.53 | 7.44 |
| Intra-Assay | 2 | 18 | 18.24 | 1.17 | 6.4 |
| Intra-Assay | 3 | 18 | 3.1 | 0.2 | 6.35 |
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